Journal: bioRxiv
Article Title: Human DCM-time machine unravels cell state changes during primitive gut tube differentiation
doi: 10.1101/2025.09.26.678757
Figure Lengend Snippet: a . Genome browser view of DCM-labelled enhancer regions, including iPSC enhancers upstream of SOX2 (left) and both iPSC- and DE-specific enhancers on chromosome 10 (right). Tracks show iPSC and DE H3K27ac ChIP-seq enrichment and corresponding significant peaks (in red), and DCM scores of iPSC dox and DE dox (average of n = 3) with significant DCM-labelled enhancers below (in blue and yellow, respectively). Note that only intergenic enhancers are called from the DCM data, meaning no enhancers overlapping with SOX2 are identified. b . Genome browser view of DCM-labelled enhancer regions, including DE enhancers upstream of GATA4 (left) and in an intergenic region on chromosome 2 (right). Tracks shown correspond to those in a. c . Heatmaps showing enrichment of DCM scores in iPSC −dox , iPSC dox and DE dox , as well as H3K27ac ChIP-seq datasets from iPSC and DE, at DCM-labelled enhancers specific to iPSCs (top) and DE (bottom). Profile plots have the same y-axis range as the corresponding heatmap. d . Significantly enriched motifs in iPSC (top) and DE (bottom) enhancer regions. Barplots show significance values reported by HOMER, with bars colored by the percentage of enhancers containing the motif sequence. Black boxes indicate whether the motif is significantly enriched in H3K27ac peaks of the corresponding cell type.
Article Snippet: Sequencing libraries were prepped after LpnPI digestion using the ThruPLEX DNA-Seq HV kit (Takara, R400740).
Techniques: ChIP-sequencing, Sequencing